Research Publications

A new biological recovery approach for PHA using mealworm, Tenebrio molitor

Bacterial polyhydroxyalkanoates (PHA) are expensive partly due to the recovery and purification processes. Thus, many studies have been carried out in order to minimize the cost.

Green mode synthesis of silver nanoparticles using Vitis vinifera’s tannin and screening its antimicrobial activity/apoptotic potential versus cancer cells

A green synthesis was employed to prepare silver nanoparticles via reduction of silver nitrate, by utilize Vitis vinifera fruit skin extract as reductant.

Influence of freeze-drying and spray-drying preservation methods on survivability rate of different types of protectants encapsulated Lactobacillus acidophilus FTDC …

The aims of this study were to compare the effectiveness of different drying methods and to investigate the effects of adding a series of individual protectant such as skim milk, sucrose, maltodextrin, and corn starch for preserving Lactobacillus acidophilus FTDC 3081 cells during spray and freeze-drying and storage at different temperatures.

Composite properties and biodegradation of biologically recovered P (3HB-co-3HHx) reinforced with short kenaf fibers

A compression-molded green composite material comprising of bacterially synthesized poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] and short kenaf fibers (KF) was prepared in the present study.

Biosynthesis of P (3HB-co-3HHx) with improved molecular weights from a mixture of palm olein and fructose by Cupriavidus necator Re2058/pCB113

A combination of palm olein (POl) and fructose was used as carbon source for the biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] by recombinant Cupriavidus necator Re2058/pCB113.

Banana frond juice as novel fermentation substrate for bioethanol production by Saccharomyces cerevisiae

Bioethanol has been produced from sugars originating from starchy staple crops such as wheat, sugarcane or corn. 

Investigation of potential anti-urolithiatic activity from different types of Musa pseudo-stem extracts in inhibition of calcium oxalate crystallization

Almost all parts of a banana tree, including the fruits, stem juice, and flowers are commonly used as traditional medicine for treating diarrhoea (unripe), menorrhagia, diabetes, dysentery, and antiulcerogenic, hypoglycemic, antilithic, hypolipidemic conditions, plus antioxidant actions, inflammation, pains and even snakebites.

Mealworm (Tenebrio molitor) oil characterization and optimization of the free fatty acid pretreatment via acid-catalyzed esterification

The physical and chemical properties of mealworm (Tenebrio molitor) oil were studied. Mealworm powder has a high oil content of 37.54 ± 0.78% with a high free fatty acid (FFA) content of 10.84 ± 0.005%.

Development and evaluation of controlled release fertilizer using P (3HB-co-3HHx) on oil palm plants (nursery stage) and soil microbes

The scope of this study was to formulate polyhydroxyalkanoate (PHA)-based controlled release fertilizer (PHA-CRF) for oil palm (nursery stage) by using biologically recovered poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) and oil palm trunk vascular bundles combined with commercial NPK fertilizer.

Production of P (3HB-co-4HB) copolymer with high 4HB molar fraction by Burkholderia contaminans Kad1 PHA synthase

Burkholderia sp. has been reported to synthesize polyhydroxyalkanoates (PHAs) with different monomers such as 3-hydroxybutyrate and 3-hydroxyvalerate. In this study, the PHA synthase gene of B. contaminans Kad1 (phaCBcon) was successfully cloned and characterized.

Response surface methodology optimization of polyhydroxyalkanoate production by Burkholderia cepacia BPT1213 using waste glycerol from palm oil‐based

The feasibility of using waste glycerol from the biodiesel industry for biosynthesis of polyhydroxyalkanoate (PHA) by Burkholderia cepacia BPT1213 was evaluated.

Production of P(3HB‐co‐3HHx) with Controlled Compositions by Recombinant Cupriavidus necator Re2058/pCB113 from Renewable Resources

Mixtures of crude palm kernel oil (CPKO) and oil palm tree trunk sap (OPTS) as carbon sources for the biosynthesis of poly(3‐hydroxybutyrate‐co‐3‐hydroxyhexanoate) (P(3HB‐co‐3HHx)) by the recombinant Cupriavidus necator strain Re2058/pCB113 were investigated.

Green Nanotechnology for Synthesis and characterization of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) nanoparticles for sustained bortezomib release using supercritical CO2

Carbon dioxide assisted particle formation combined with electrospraying using supercritical CO2 (scCO2) as an aid (Carbon Dioxide Assisted Nebulization-Electrodeposition, CAN-ED) was used to produce Bortezomib loaded poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) P(3HB-co-3HHx) nanoparticles for sustained release.

Evaluation of soil burial biodegradation behavior of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) on the basis of change in copolymer composition monitored by thermally …

Soil burial biodegradation behavior of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] was studied based on its copolymer composition determined by thermally assisted hydrolysis and methylation-gas chromatography (THM-GC).

Structural Analysis of ZnO Nanoparticles Reinforced P(3HB-co-15 mol% 3HHx) Bioplastic Composite

Bioplastics are gaining interest due to their biodegradable and biocompatible nature which can be used as a replacement for petroleum based plastics.

Biosynthesis of polyhydroxyalkanoates using Cupriavidus necator H16 and its application for particleboard production

Different concentrations of palm oil and urea were used in a preliminary experiment conducted in a shake-flask to determine the optimum conditions for the biosynthesis of polyhydroxyalkanoates (PHA). 

Validation of thermally assisted hydrolysis and methylation-gas chromatography for rapid and direct compositional analysis of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) in

Thermally assisted hydrolysis and methylation-gas chromatography (THM-GC) in the presence of an organic alkali was validated for the compositional analysis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] accumulated in whole bacterial cells.

Physicochemical Studies of Kenaf Nanocrystaline Cellulose and Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) as Filler for Lithium Perchlorate based Polymer Electrolyte

Solid state polymer electrolyte attracts a great attention due to its thermal stability, no leakage, excellent flexibility, and good processability. There are many polymers have been investigated for electrochemical device applications.

A study of antibacterial efficacy of Alpinia galangal extracts against Staphylococcus aureus, Staphylococcus epidermidis and Listeria monocytogenes

this study mainly focused on effectiveness of A. galangal against antibacterial activity of selected gram-positive bacteria which are S. aureus, S. epidermidis and L. Monocytogens.

Bioseparation of phycocyanin from Phormidium tergestinum using an aqueous two-phase system

This study aimed at purification of phycocyanin (PC) from Phormidium tergestinum using an aqueous two-phase system (ATPS) comprised of polyethylene glycol (PEG) and salts.

Isolation and characterization of Lactobacillus brevis C23 with ability to secrete antimicrobial substance for the inhibition of a foodborne pathogen Listeria monocytogenes

This study aims to isolate lactic acid bacteria (LAB) from various food sources to obtain a potent strain against Listeria monocytogenes.

Synergistic influence of flame retardant additives and citric acid on the functional properties of rice husk/wood blended particleboards

The selected functional properties of rice husk/wood blended particleboards which include thermal analysis, limiting oxygen index, morphological analysis, and mechanical properties have been investigated.

Biosynthesis of PHAs and Their Biomedical Applications

This chapter contains sections titled: Introduction Genetic and Metabolic Pathway of PHA Production PHA Production from Sugars PHA Production from Oils Exploration and Application of PHAs as Biomaterials Future Perspectives Acknowledgments

Polymer impregnation in porous glass beads to induce bioseparation of β-mannanase from fermentation broth of Proteus vulgaris

Mannanase has been used for the hydrolysis of mannan, which is produced by many microorganisms including bacteria. The purified enzyme can be utilized in the production of high-value product such as mannooligosaccharides as prebiotics. 

A new biological recovery approach for PHA using mealworm, Tenebrio molitor

Bacterial polyhydroxyalkanoates (PHA) are expensive partly due to the recovery and purification processes. Thus, many studies have been carried out in order to minimize the cost.

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